What Are the Challenges in Measuring Signal Intensity?
Measuring signal intensity accurately can be challenging due to several reasons:
Background noise: Unwanted signals or noise can interfere with the measurement of true signal intensity. Photobleaching: Prolonged exposure to light can cause fluorophores to lose their fluorescence, affecting signal intensity over time. Variability in tissue samples: Differences in tissue composition and preparation can lead to inconsistent signal intensities.
