Several factors can influence the binding efficiency of stains and labels in histology:
Tissue Preparation: Proper fixation, embedding, and sectioning of the tissue are foundational steps that impact staining quality. pH Levels: The pH of staining solutions can affect the binding affinity of dyes and antibodies to their targets. Temperature: The temperature at which staining is performed can influence the interaction between stains and tissue components. Incubation Time: Adequate incubation time is necessary to ensure that stains have sufficient time to bind to their targets. Concentration of Stains: Optimal concentrations of stains and antibodies must be used to achieve effective binding without background staining.
