How are Humectants Applied in Histological Procedures?
Humectants can be used at various stages of histological processes:
Fixation: Some fixation solutions include humectants to prevent tissue shrinkage and maintain structural integrity. Embedding: During paraffin embedding, humectants can be added to prevent tissue dehydration. Staining: Humectants are often included in staining protocols to ensure that tissues do not dry out during the staining process. Mounting: Mounting media containing humectants are used to preserve stained tissues on slides for long-term storage and examination.