Several factors can impact counting accuracy in histology:
Sample Preparation: Proper tissue processing and staining techniques are vital for clear visualization and accurate counting. Section Thickness: Inconsistent thickness of tissue sections can lead to errors in cell count due to overlapping structures. Counting Method: Manual counting under a microscope versus automated counting systems can yield different levels of accuracy. Human Error: Variability between observers and fatigue can introduce inaccuracies in manual counting. Image Quality: Poor quality images can obscure details necessary for accurate cell identification and counting.
