Both manual and automated counting methods have their advantages and limitations:
Manual Counting:
Advantages: Allows for direct observation and assessment of tissue morphology. Useful for small sample sizes and specialized analyses. Limitations: Time-consuming, prone to human error, and less reproducible.
Automated Counting:
Advantages: High throughput, consistent, and less subjective. Capable of handling large datasets efficiently. Limitations: May require expensive equipment and software. Can struggle with complex or poorly stained samples.