Variability in Reagent Quality: The quality and concentration of staining reagents can vary between batches, leading to inconsistent results. Fixation Issues: Improper fixation of tissue samples can result in uneven penetration of stains, affecting the visibility of cellular structures. Technique Variations: Differences in staining protocols, such as incubation times and temperatures, can cause variability. Human Error: Manual handling of samples and reagents introduces the potential for errors that can affect staining outcomes.
