There are several transfection methods, each with its own advantages and limitations:
1. Chemical Transfection: Utilizes chemical reagents like calcium phosphate, DEAE-dextran, and liposomes to facilitate the entry of nucleic acids into cells. 2. Physical Transfection: Methods such as electroporation and microinjection physically introduce nucleic acids into cells by creating transient pores in the cell membrane. 3. Viral Transfection: Uses viral vectors to deliver genetic material into cells. This method is highly efficient and often used for stable transfections.
