What are the Challenges in Transfection for Histological Studies?
Despite its utility, transfection in histology presents several challenges:
- Cell Type Specificity: Different cell types have varying transfection efficiencies, requiring optimization of methods for each cell type. - Toxicity: Some transfection reagents can be toxic to cells, affecting their viability and altering experimental outcomes. - Transient vs. Stable Transfection: Achieving stable transfection, where the introduced gene is incorporated into the genome, is more challenging than transient transfection.
