Enzyme reactions are visualized using various histochemical techniques. The most common methods include:
- Colorimetric Methods: These methods involve the use of chromogenic substrates that produce a colored product upon reaction with the enzyme. The colored product is then visualized under a light microscope. - Fluorometric Methods: These methods use fluorogenic substrates that produce a fluorescent product upon reaction with the enzyme. The fluorescent product is visualized using a fluorescence microscope. - Electron Microscopy: In some cases, enzyme reactions are visualized using electron-dense substrates that can be observed under an electron microscope. This method provides detailed information about the subcellular localization of enzyme activity.
