There are several types of multiplex staining techniques, including:
Fluorescent Multiplex Staining: Utilizes distinct fluorescent dyes to label different targets. The dyes emit light at different wavelengths when excited, allowing for simultaneous detection. Chromogenic Multiplex Staining: Uses enzymes that react with chromogenic substrates to produce differently colored precipitates, enabling the visualization of multiple targets. Multiplex Immunohistochemistry (IHC): Combines antibodies conjugated with different labels to detect specific antigens within a tissue section. In Situ Hybridization (ISH): Employs labeled probes to detect specific nucleic acid sequences, often used in combination with other staining methods.
