What are the Steps Involved in Gomori's Reticulin Staining?
The process typically involves the following steps:
Oxidation: The tissue is treated with an oxidizing agent like potassium permanganate to expose aldehyde groups. Sensitization: The tissue is then treated with ferric ammonium sulfate to enhance the deposition of silver. Silver Impregnation: Silver nitrate is applied to the tissue, forming a silver complex with the aldehyde groups. Reduction: The silver complex is reduced to metallic silver using a reducing agent like formalin, making the reticular fibers visible. Toning: Gold chloride is used to enhance the contrast and quality of the staining. Fixation: Sodium thiosulfate is applied to remove any unreacted silver, stabilizing the stain.
