The production of recombinant proteins involves several steps: 1. Gene Cloning: The gene encoding the target protein is isolated and inserted into a suitable vector. 2. Transformation: The recombinant vector is introduced into a host organism, such as bacteria, yeast, or mammalian cells. 3. Expression: The host cells are cultured under conditions that promote the expression of the recombinant protein. 4. Purification: The expressed protein is purified from the host cells using techniques like affinity chromatography.
