What are the Types of Labels Used in Immunocytochemistry?
Several types of labels can be used in ICC to detect the binding of antibodies to antigens. These include:
Fluorescent dyes: These are commonly used for their high sensitivity and ability to provide multi-color imaging. Enzymes: Enzymes such as horseradish peroxidase (HRP) or alkaline phosphatase (AP) can catalyze colorimetric reactions, making them useful for bright-field microscopy. Gold particles: These can be used for electron microscopy, offering high-resolution images of antigen localization.