What are the Techniques Used to Study Cytokines in Histology?
Several techniques are employed to study cytokines in histology:
Immunohistochemistry (IHC): This technique uses antibodies to detect specific cytokines in tissue sections. In Situ Hybridization (ISH): This method detects cytokine mRNA within tissue sections, providing insights into gene expression. Flow Cytometry: This technique can analyze the expression of cytokines in individual cells, often used in conjunction with cell surface markers. Enzyme-Linked Immunosorbent Assay (ELISA): This is used to quantify cytokine levels in tissue extracts or cell culture supernatants. Western Blotting: This method detects specific cytokine proteins in tissue extracts, providing information on protein expression levels.
