What are the Techniques Used to Study Cytokines in Histology?

Several techniques are employed to study cytokines in histology:

Immunohistochemistry (IHC): This technique uses antibodies to detect specific cytokines in tissue sections.
In Situ Hybridization (ISH): This method detects cytokine mRNA within tissue sections, providing insights into gene expression.
Flow Cytometry: This technique can analyze the expression of cytokines in individual cells, often used in conjunction with cell surface markers.
Enzyme-Linked Immunosorbent Assay (ELISA): This is used to quantify cytokine levels in tissue extracts or cell culture supernatants.
Western Blotting: This method detects specific cytokine proteins in tissue extracts, providing information on protein expression levels.