ziehl neelsen staining

What are the Steps Involved in Ziehl-Neelsen Staining?


Preparation: A sample smear is prepared and heat-fixed onto a slide.
Primary Staining: The slide is flooded with carbol fuchsin and heated gently to facilitate dye penetration.
Decolorization: The slide is washed with acid-alcohol to remove the dye from non-acid-fast cells.
Counterstaining: Methylene blue is applied to stain non-acid-fast cells and background tissues.
Observation: The slide is examined under a microscope. Acid-fast organisms appear red, while non-acid-fast organisms and background tissue appear blue.

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