Several processes in histology necessitate stringent temperature control:
Fixation: Optimal temperatures are essential to preventing autolysis and preserving tissue architecture. Embedding: Paraffin wax embedding requires precise temperatures for proper infiltration of tissues. Sectioning: Cryostat sectioning and microtomy must be performed at controlled temperatures to achieve thin, uniform sections. Staining: Many staining protocols require specific temperatures to ensure proper binding and visibility of stains.
