Which Processes Require Temperature Control?

Several processes in histology necessitate stringent temperature control:

Fixation: Optimal temperatures are essential to preventing autolysis and preserving tissue architecture.
Embedding: Paraffin wax embedding requires precise temperatures for proper infiltration of tissues.
Sectioning: Cryostat sectioning and microtomy must be performed at controlled temperatures to achieve thin, uniform sections.
Staining: Many staining protocols require specific temperatures to ensure proper binding and visibility of stains.