Fluorescent calcium indicators typically consist of a dye that binds to calcium ions. Upon binding, the fluorescence properties of the dye change, either by increasing in intensity or shifting in wavelength. This change can be detected using fluorescence microscopy or spectroscopy. The most commonly used indicators include Fura-2, Fluo-3, and Rhod-2. Each of these indicators has unique properties that make them suitable for different types of experiments.
