What Are the Steps Involved in Immediate Fixation?
1. Tissue Harvesting: The tissue is promptly removed from the organism with minimal trauma. 2. Immersion in Fixative: The tissue is immediately submerged in an appropriate fixative solution. 3. Optimal Conditions: Factors like temperature, pH, and osmolarity are controlled to enhance fixation efficacy. 4. Duration of Fixation: The tissue is left in the fixative for a period, typically ranging from a few hours to overnight, depending on the fixative and tissue type. 5. Post-Fixation Processing: After fixation, the tissue is washed to remove excess fixative and then processed for further histological examination.
