Different stages of histological preparation have specific temperature requirements:
Fixation: Generally performed at room temperature (~20-25°C), but some protocols may require refrigeration. Embedding: Paraffin embedding requires temperatures around 56-58°C to melt the wax without damaging the tissue. Sectioning: Microtomy is usually done at slightly cooler temperatures (~4-10°C) to ensure the paraffin is firm. Staining: Some staining protocols require incubation at specific temperatures, often around 37°C.
