The basic steps of autoradiography include: 1. Labeling: The biological molecules of interest are labeled with a radioactive isotope. 2. Incorporation: The labeled molecules are introduced into the tissue sample. 3. Sectioning: The tissue is sectioned into thin slices for analysis. 4. Exposure: The tissue sections are placed on a photographic film or emulsion and exposed to the radioactive emissions. 5. Development: The film is developed to reveal the pattern of silver grains, indicating the location of the labeled molecules.
