What are the Limitations of Using Ct in Histology?
While Ct values provide valuable quantitative data, there are limitations to their use in histology. One limitation is the potential for PCR inhibition due to contaminants in tissue samples, which can lead to inaccurate Ct values. Additionally, the requirement for high-quality RNA may be challenging to achieve in some histological samples, particularly those that are archived or have undergone extensive processing. Moreover, Ct values alone do not provide information about the localization of gene expression within tissues, which is often critical in histological analysis.
