The Threshold Cycle (Ct) is a critical parameter in quantitative PCR (qPCR) that indicates the cycle number at which the fluorescence signal of the PCR product exceeds a defined threshold. This threshold is set above the background noise and is crucial for determining the relative abundance of target nucleic acids in various samples. In the context of Histology, Ct values help in quantifying gene expression levels in tissue samples, providing insights into cellular functions and pathological states.
