What are the Limitations of Using CD25 as a Marker?
While CD25 is a valuable marker, it has limitations. Its expression is not exclusive to Tregs and activated T cells, making it challenging to distinguish between different cell types solely based on CD25. Moreover, the expression levels of CD25 can vary, and not all activated T cells or Tregs may express CD25 at detectable levels, potentially leading to underestimation of these cell populations in certain conditions.
