Despite its advantages, the MTT assay has some limitations: - Interference: Some compounds can interfere with the MTT reduction process, leading to inaccurate results. - Non-specificity: The assay does not distinguish between different types of cell death (e.g., apoptosis vs. necrosis). - Cell Density: The assay’s accuracy can be affected by the density of the cell culture, as overly confluent or sparse cultures may give misleading results. - [Endpoint Measurement](): The assay only provides an endpoint measurement, and does not offer real-time data on cell viability.
