The MTT assay typically involves the following steps: 1. [Cell Seeding](): Cells are seeded in a 96-well plate and allowed to adhere and grow. 2. Treatment: Cells are treated with the test compound or condition. 3. MTT Addition: MTT reagent is added to each well and incubated for a specified period. 4. Formazan Solubilization: After incubation, a solubilizing agent is added to dissolve the formazan crystals. 5. Absorbance Measurement: The absorbance of each well is measured using a spectrophotometer.
