Fixation: Tissue samples are fixed using formaldehyde to preserve cellular structures. Sectioning: The fixed tissue is sectioned into thin slices using a microtome. Staining: The tissue sections are stained with a basic dye such as cresyl violet or toluidine blue. Dehydration: The stained sections are dehydrated using alcohol and cleared in xylene. Mounting: The sections are mounted on glass slides and covered with a coverslip for microscopic examination.
