high resolution microscopy

What are the Limitations of High Resolution Microscopy?

Despite its advantages, high resolution microscopy has some limitations:
Cost: High-end microscopy equipment and maintenance can be very expensive.
Sample Preparation: Requires meticulous preparation, which can be time-consuming and technically demanding.
Artifact Formation: The processes involved in preparing samples can sometimes introduce artifacts that may complicate interpretation.
Limited Penetration: Techniques like electron microscopy are limited to very thin sections of tissue, which may not provide a complete picture of the three-dimensional structure.

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