Staining techniques are essential for enhancing the contrast in tissue samples. Different stains can highlight various cellular components. For instance, Hematoxylin and Eosin (H&E) is a commonly used stain that differentiates between the nucleus and cytoplasm. However, other stains like Periodic Acid-Schiff (PAS) and Masson's Trichrome are used for specific purposes, and their interpretation can vary based on the stain applied.