What are the Limitations of Enhanced Resolution Techniques?

Despite their advantages, enhanced resolution techniques have limitations:

Cost: High-resolution microscopes and related equipment can be expensive, making them less accessible to some laboratories.
Sample preparation: Some techniques require complex and time-consuming preparation, which can be a barrier for routine use.
Technical expertise: Advanced techniques often require specialized knowledge and training to operate effectively.
Compatibility: Not all enhanced resolution methods are compatible with all types of samples or staining protocols.