The quantification and analysis of biomarker data involve several steps:
Image Acquisition: High-resolution images of stained tissue sections are acquired using microscopes equipped with cameras. Image Processing: Images are processed to enhance contrast and remove background noise. Segmentation: The tissue sections are segmented to identify regions of interest (ROIs) where biomarkers are to be quantified. Quantification: The intensity and distribution of signals within the ROIs are measured using specialized software. Statistical Analysis: Quantified data are analyzed statistically to draw meaningful conclusions.
