Several techniques have been developed to improve resolution in histological studies:
Confocal microscopy: This technique uses laser light to scan specimens, producing high-resolution images by eliminating out-of-focus light. Super-resolution microscopy: Techniques like STED, PALM, and STORM break the diffraction limit of light, providing resolution beyond conventional light microscopy. Electron microscopy: Using electrons instead of light waves, this method offers extremely high resolution, allowing visualization of ultrastructural details. Multiphoton microscopy: This advanced form of fluorescence microscopy enables deep tissue imaging with reduced phototoxicity.
