What are the Limitations of Bioluminescence Imaging?
Despite its advantages, BLI has some limitations:
Tissue penetration: The light emitted by luciferase can be absorbed or scattered by tissues, limiting the depth of imaging. Substrate dependency: The availability of luciferin is crucial for the bioluminescence reaction, and its delivery can be challenging in some cases. Background noise: Background bioluminescence from endogenous sources can interfere with the signal, reducing the signal-to-noise ratio.
