Despite its powerful capabilities, patch clamp recording has several challenges:
1. Technical Skill: The technique requires a high level of expertise and manual dexterity to achieve and maintain a gigaseal. 2. Cell Viability: Prolonged recordings can be challenging as cells may deteriorate over time, affecting the quality of data. 3. Complex Data Analysis: The data obtained from patch clamp experiments can be complex and requires sophisticated analysis tools and methods.
