What are the Limitations of 2D Histological Techniques?
Despite their widespread use, 2D histological techniques have certain limitations:
Loss of 3D Context: These techniques provide a two-dimensional view, which can limit the understanding of complex three-dimensional structures. Sample Preparation Artifacts: The process of fixation, embedding, and sectioning can introduce artifacts that may alter the tissue's natural state. Limited Molecular Information: Traditional staining techniques may not provide detailed molecular information, necessitating the use of more advanced methods like IHC or in situ hybridization.
