What are the Limitations and Challenges of Fluorometric Assays?
Despite their advantages, fluorometric assays have some limitations and challenges:
- Photobleaching: Prolonged exposure to the excitation light can cause fluorescent dyes to lose their ability to emit light, leading to photobleaching. - Autofluorescence: Some biological tissues exhibit autofluorescence, which can interfere with the detection of specific fluorescent signals. - Background Noise: Non-specific binding of fluorescent dyes can create background noise, complicating the interpretation of results. - Cost: The equipment and reagents required for fluorometric assays can be expensive.
