What are the Common Applications of Fluorometric Assays in Histology?
Fluorometric assays are used in a wide range of histological applications:
- Immunofluorescence: This technique involves using fluorescently labeled antibodies to detect specific proteins within tissue sections. - FISH (Fluorescence In Situ Hybridization): A method used to detect and localize specific DNA sequences within chromosomes. - Live-Cell Imaging: Fluorometric assays enable the visualization of dynamic processes in living cells, such as protein interactions and cellular movements. - Apoptosis Detection: Assays like TUNEL (Terminal deoxynucleotidyl transferase dUTP nick end labeling) use fluorescence to identify apoptotic cells by labeling DNA breaks.
