1. Sample Collection and Fixation: The initial step involves obtaining a quality tissue sample. This must be done carefully to avoid damaging the tissue. Fixation, which preserves the tissue, often requires several hours to days.
2. Embedding and Sectioning: Embedding the tissue in paraffin or another medium and then sectioning it into thin slices are tasks that demand steady hands and a careful approach to avoid tearing or distorting the tissue.
3. Staining: Different stains are used to highlight various cellular components, and this process can be time-consuming. Each stain has its own protocol that must be followed meticulously.
