Under a microscope, coagulative necrosis can be identified by the presence of ghostly outlines of cells in the affected tissue. The cells appear eosinophilic (pink) on Hematoxylin and Eosin (H&E) staining, and the nuclei are often fragmented or completely absent, a condition known as karyolysis. The cytoplasm becomes more homogenous and glassy, due to the loss of cellular organelles.
