The DNase-seq process involves several critical steps:
Tissue Collection: Samples are collected from the tissue of interest. Chromatin Preparation: Cells are lysed, and chromatin is isolated. DNase I Digestion: The isolated chromatin is treated with DNase I, which specifically digests open and accessible regions of DNA. DNA Purification: The digested DNA fragments are purified. Library Preparation and Sequencing: The purified DNA is used to prepare sequencing libraries, which are then sequenced using high-throughput sequencing technologies. Data Analysis: Sequencing reads are mapped to the reference genome, and regions of high read density are identified as DNase I hypersensitive sites.
