The tissue preparation process involves several key steps, each of which must be meticulously executed to ensure high quality. These steps include:
1. Fixation: This step preserves tissue morphology and prevents autolysis and decay. 2. Processing: Dehydration, clearing, and infiltration with a embedding medium like paraffin. 3. Embedding: Encasing the tissue in a solid medium for sectioning. 4. Sectioning: Cutting thin slices of tissue for placement on slides. 5. Staining: Applying dyes to enhance contrast and highlight specific structures.
