Tissue processing involves dehydration, clearing, and infiltration. Common issues include:
- Incomplete Dehydration: Residual water can cause poor infiltration of embedding media, leading to soft, mushy sections. - Inadequate Clearing: Insufficient clearing can result in opaque sections, making microscopic examination difficult. - Improper Infiltration: Poor infiltration can cause uneven embedding, which complicates sectioning and staining.
