What are the Key Steps in Routine Histological Procedures?
The main steps involved in routine histological procedures include:
Fixation: Preserving the tissue to prevent degradation and maintain cellular structure. Processing: Dehydrating, clearing, and infiltrating the tissue with a medium (usually paraffin) to prepare it for sectioning. Embedding: Encasing the tissue in a solid medium to provide support during sectioning. Sectioning: Cutting thin slices of the embedded tissue using a microtome. Staining: Applying dyes to enhance the visibility of cellular components under a microscope. Mounting: Placing the stained sections on slides and covering them with a coverslip for examination.
