1. Cell Lysis: Tissue samples are lysed to release intracellular proteins. 2. Incubation with Antibody: An antibody specific to the bait protein is added to the lysate. 3. Binding to Beads: Protein A/G beads are introduced to capture the antibody-protein complexes. 4. Washing: Non-specifically bound proteins are washed away. 5. Elution: The antibody-protein complexes are eluted from the beads. 6. Analysis: The eluted proteins are analyzed, commonly by Western blotting or mass spectrometry.
