What are the Histological Techniques for Studying Ischemia?
Several histological techniques are employed to study ischemic tissues:
Hematoxylin and Eosin (H&E) Staining: The primary method for examining cellular and tissue morphology in ischemic conditions. Immunohistochemistry: Used to detect specific proteins, such as markers of apoptosis or inflammation, providing insights into the molecular events during ischemia. Electron Microscopy: Offers detailed visualization of ultrastructural changes in cells, such as mitochondrial damage and membrane disruptions. TUNEL Assay: Identifies DNA fragmentation, a hallmark of apoptosis, in ischemic tissues.
