What are the histological methods used to study PYY?
Histological techniques used to study PYY include: - Immunohistochemistry (IHC): This technique utilizes antibodies specific to PYY to visualize its presence and distribution in tissue sections. It helps in identifying the cells that produce PYY and their localization within the gastrointestinal tract. - In Situ Hybridization (ISH): ISH is used to detect PYY mRNA in tissue samples, providing insights into the gene expression patterns of PYY. - Electron Microscopy: This allows for the ultrastructural examination of L-cells and the secretory granules containing PYY, offering detailed visualization at the cellular level.
