1. Tissue Preparation: Fixing and embedding the tissue, followed by sectioning. 2. Probe Preparation: Designing and labeling the probe. 3. Hybridization: Incubating the tissue sections with the probe under conditions that promote specific binding. 4. Washing: Removing unbound probes through a series of washes. 5. Detection: Visualizing the bound probe using appropriate detection methods, such as fluorescence or enzymatic reactions. 6. Analysis: Imaging and quantifying the hybridization signals.
