The process of creating 2D representations involves several steps: 1. Fixation: The tissue is preserved using chemicals like formaldehyde to prevent degradation. 2. Embedding: The fixed tissue is embedded in a medium such as paraffin to provide support for thin sectioning. 3. Sectioning: Thin slices of the tissue, usually 3-5 micrometers thick, are cut using a microtome. 4. Staining: The sections are stained with dyes like hematoxylin and eosin (H&E) to differentiate various cellular components. 5. Microscopy: The stained sections are examined under a light microscope to produce high-resolution 2D images.
