What Are the Considerations When Using β Galactosidase in Histological Studies?
When employing β Galactosidase in histological studies, researchers must consider several factors:
Substrate Specificity: Choosing the appropriate substrate is crucial for the sensitivity and specificity of the assay. Tissue Preparation: Proper tissue fixation and embedding techniques are necessary to preserve β Galactosidase activity and enable accurate detection. Controls: Including positive and negative controls in experiments ensures the reliability and reproducibility of results.