What are the Common Challenges in Protein Identification?
Several challenges can arise during protein identification in histology:
1. Antibody Specificity: Ensuring that the antibodies used are specific to the target protein and do not cross-react with other proteins is crucial.
2. Tissue Preservation: Proper fixation is essential to preserve protein structure and prevent degradation. Over-fixation or under-fixation can obscure results.
3. Background Staining: Non-specific binding of antibodies can lead to high background staining, making it difficult to interpret the results.
4. Quantification: Quantifying protein levels in tissue sections can be challenging, especially when using colorimetric detection methods.
