What are the Challenges of Fluorescent Multiplexing?
Despite its advantages, fluorescent multiplexing comes with certain challenges:
Spectral overlap: The emission spectra of different fluorophores can overlap, leading to signal bleed-through and complicating data interpretation. Photobleaching: Prolonged exposure to light can cause fluorophores to lose their fluorescence, affecting the quality of the data. Optimization: Careful optimization of staining protocols and imaging parameters is required to achieve reliable and reproducible results.
